IHSS&IWA26 / BRNO / CZECHIA / 23–28 August 2026 BOOK OF ABSTRACTS 218 Poster Session / Environment, Agriculture, and Forestry Hall A To achieve these goals, an integrative methodology has been developed, combining controlled laboratory incubation experiments with semifield mesocosm trials to reflect realworld agricultural conditions across diverse soil textures (sands, loams, and silty loams). Pesticide concentration dynamics are monitored using an optimized QuEChERS-based extraction protocol followed by high-sensitivity LC-MS/MS analysis. A key novelty of the project is the simultaneous assessment of soil biological integrity through enzymatic profiling (dehydrogenase and β-glucosidase activities) and advanced ecotoxicological assays. These include the Microbial Assay for Risk Assessment (MARA), Microtox and multi-species invertebrate tests involving Eisenia fetida and Folsomia candida. By identifying the mechanistic synergies between plant-derived antioxidants and pesticide degradation pathways, this research aims to establish new directions for sustainable rhizosphere management and the development of „green“ strategies to enhance soil self-purification processes. The expected outcomes will provide practical guidelines for farmers and horticulturalists on using specific plantbased soil amendments or crop rotations to naturally accelerate the detoxication of agricultural land. Ultimately, this approach will support the transition towards circular bioeconomy and safer food production by minimizing the chemical burden in soil-plant systems. Acknowledgement This work was supported by the National Science Centre, Poland, under the project OPUS 29, grant no. 2025/57/B/ ST10/01727, entitled „Influence of secondary plant metabolites on degradation of pesticides in agricultural soils“ (2026–2029).
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