IHSS&IWA26 / BRNO / CZECHIA / 23–28 August 2026 BOOK OF ABSTRACTS 222 Poster Session / Environment, Agriculture, and Forestry Hall A these soil applications. During the experiment, soil pH dynamics and agronomic parameters of the plants were monitored. After the termination of plant cultivation experiment, a comprehensive analysis of the soil, plant this soil application activity in the rhizosphere using the BIOLOG EcoPlate™ kit was performed. Specific PGPR effects were tested as well, including nitrogen fixation, phosphate solubilization, and the production of siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and indole-3-acetic acid (IAA). The obtained results indicate that the combination of biochar with PGPR has the potential to enhance the effects of biochar in soil applications, particularly in terms of pH stabilization and support for microbial activity in the rhizosphere. However, since these are preliminary experiments conducted within complex matrices, it would be necessary to repeat the realized measurements with a higher number of replicates to obtain statistically more significant results and subsequently verify the obtained outcomes in semi-field experiments under real-soil conditions. References 1. P. R. Yaashikaa, P. S. Kumar, S. Varjani, A. Saravanan, Biotechnology Reports 28 (2020) e00570. 2. H. Wu, Y. Huo, F. Qi, Y. Zhang, R. Li et al., Biochar 7 (2025) 113
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